Talking about objective lens and eyepiece

When choosing objective and eyepieces, two main issues are considered:

First, match on category. That is, all flat field objectives must be used in conjunction with special flat field eyepieces.

Second, reasonable matching of magnification. Under a certain magnification, the objective lens and eyepiece can be arbitrarily combined, but the premise of the combination is mainly to consider the effective magnification, which is an important rule for the correct use of the microscope (Microscope). The effective magnification of the microscope (Microscope) is 500 to 1000 times the numerical aperture of the objective lens used, that is, the product of the magnification of the objective lens and the eyepiece is equal to 500 to 1000 times the numerical aperture of the objective lens.

If you use a 40X objective lens with a numerical aperture of 0.65 to observe the specimen, what size should you choose?

First, the effective total magnification is calculated according to the effective magnification, and then divided by 40, that is, the magnification of the eyepiece should be selected. The calculation process is: (0.65 × 500 ~ 0.65 × 1000) ÷ 40≈8 ~ 16. That is to say: the objective lens with a numerical aperture of 0.65 should be matched with an eyepiece of 8 to 16 times in the range of effective magnification (500 to 1000 times). If the eyepiece magnification is too low, the total magnification is too small, and the resolution of the objective lens cannot be fully exerted. The details that could have been recognized can be difficult to distinguish because the total magnification is too small. The magnification obtained by eyepieces higher than 16 times is called "empty magnification". The resolution of the affected details is not improved at all, but the clear depth is small, and it cannot clearly reflect the structure of different levels.

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