Size and location of oxygen flow rate during the development of Neurospora crassa mycelium
The mechanism of mycelial development is relatively complicated, and the fungal hyphae provide a good material for the study of hyphae due to the clear morphological structure. The ion gradient formed by the Ca2 + ion current plays an important role in the regulation of mycelial development. A distinctive feature of Neurospora crassa is that the tip of the mycelium is rich in mitochondria, providing ATP for mycelial development. In order to study the respiratory activity of the mitochondria at the tip of the mycelium, a high spatial resolution oxygen flow measurement method is needed. Canadian researcher Roger R. Lew used Neurospora crassa as the experimental material, and used non-damaging micro-measurement technology combined with micro-technology to study the oxygen flow rate changes in different regions of the mycelium surface after cyanide treatment. It was found that the rate of oxygen influx (absorption) on the surface of the mycelium can be suppressed by cyanide, while alternating oxidase can restore respiration. There is a region with a large oxygen influx rate 10 μm behind the tip of the mycelium, and the rate of oxygen influx is related to the growth rate of the mycelium, while the area where mitochondria are enriched on the mycelium is 5-10 μm from the tip It is different from the area of ​​maximum oxygen inflow. Therefore, the mitochondria at the tip of the mycelium play a smaller role in the respiratory requirements of growth and development. Based on the above results, they believe that mitochondria enriched in tips may play a role in removing cytoplasmic Ca2 +, and speculate that the tip of mycelium may be the site of mitochondrial occurrence. Keywords: Oxygen flux mapping; Hyphal growth; Self-referencing technique; Neurospora crassa References: Lew, RR and Levina, NN FEMS Microbiology Letters, 2004, 233: 125-130. Two Function Bed,Multi Functional Sickbed,2 Function Hospital Bed,Medical Bed For Home Hebei Boxin Rehabilitation Equipment Co., Ltd , https://www.boxinnursingbed.com