Human C-type natriuretic peptide (CNP) kit instruction manual

Detection range: 96T0.2pg/ml-8pg/ml Use purpose: This kit is used to determine the content of C-type natriuretic peptide (CNP) in human serum, plasma and related liquid samples. Experimental principle The kit uses a double antibody sandwich method to determine the level of human C-type natriuretic peptide (CNP) in the specimen. The microporous plate was coated with purified human C-type natriuretic peptide (CNP) antibody to prepare a solid phase antibody, and C-type natriuretic peptide (CNP) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled C. The natriuretic peptide (CNP) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added with the substrate TMB. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the C-type natriuretic peptide (CNP) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human C-type natriuretic peptide (CNP) in the sample was calculated from a standard curve.

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